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Draw volume = 330 mcg 3,333 mcg/mL = 0.099 mL

1 Remove AOD-9604 vial from refrigeration and allow to reach room temperature (1520 minutes) 2 Clean vial top with alcohol swab and allow to air dry completely 3 Using a sterile syringe, draw the calculated volume of bacteriostatic water (BAC water) 4 Inject BAC water slowly down the side of the vial do not aim directly at the powder 5 Gently swirl in circular motions DO NOT shake vigorously as this degrades the peptide 6 Allow 23 minutes for full dissolution solution should be clear and colorless 7 Draw calculated dose into an insulin syringe for subcutaneous injection 8 Inject into abdomen, thigh, or upper arm

Conclusions These results demonstrate that gene-expression changes associated with regional emphysema severity within an individual's lung can provide insights into emphysema pathogenesis and identify novel therapeutic opportunities for this deadly disease

Clinical use: Supervised protocols at medical clinics involve subcutaneous injection over 4 to 8 weeks, with dosing individualized to injury type and severity

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